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Laboratory of Molecular Image on Translational Research

第十八屆國家新創獎得獎研究主題分享
精準免疫幹細胞療法:雙基因修飾臍帶間質幹細胞治療實體癌症

此雙基因表現間質幹細胞可同時遞送程序性死亡受體(PD-1)與HSV-1-TK基因。此雙基因修飾間質幹細胞搭配更昔洛韋(ganciclovir)的注射,可啟動旁觀者效應來達到毒殺癌細胞之效果,並透過間質幹細胞中表現PD-1之特性,使其與癌細胞上表現之PD-L1結合,防止癌細胞與效應型T細胞 (effector T cells) 受腫瘤免疫抑制機制所影響,以維持效應型T細胞之免疫攻擊性。經基因工程改造後可使臍帶間質幹細胞能夠更全方位的將癌細胞毒殺。

Immune precision medicine: Development of dual genetic engineering umbilical cord mesenchymal stem cells in immunotherapy for solid tumor
The innovation of this application is that we apply gene engineering UMSCs on cancer therapy. Our group used "non-viral transfer technology" to establish "dual gene" expression UMSCs, which composed with suicide gene herpes simplex virus thymidine kinase (HSV-TK) and programmed death receptor 1 (PD-1). Through the bystander effect and the distant systemic immune response which induced by the suicide gene HSV-TK combined with drug ganciclovir (GCV), the death signal of itself and their surrounding tumor cells and immunosuppression signal were be activated. The activation of cell death signal in UMSCs can also prohibit the undesirable effect of the retention within tissue. Moreover, through the interaction of programmed death receptor 1 and programmed death ligand 1 (PD-L1) on tumor, it acts as an immune checkpoint inhibitor to weaken the immunosuppressive microenvironment around the tumor. UMSCs may precisely deliver therapeutic genes to cancer cells, followed with the activation of therapeutic genes by combining GCV to achieve precision medicine.

research resources

Main resources and equipment in LMITR

Our lab equip with following equipment including luminescence fluorescent imaging system (SpectraMax iD3), mouse surgical microscope platform and system, biomedical image analysis software (PMOD), luminescence imaging system (UVP ChemiDoc-It imaging system), Flow Cytometry (ACEA biosciences. Inc. NOVOCYTE), tissue homogenization system (gentleMACS™ Dissociator)... etc. The above-mentioned equipment can be used for the application of nanomaterials in cancer treatment and biomedical imaging.

Cooperators:

  1. Center for Advance Molecular Imaging and Translation, Chang Gung Memoiral Hospital, New Taipei City

  2. China Medical University Hospital, Taichung

  3. Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan City

  4. Taipei Medical University, Taipei

  5. National Cheng Kung University, Tainan

  6. National Yang Ming Chiao Tung University, Taipei

  7. The University of Melbourne, Australia

  8. Centre For Eye Research Australia

 

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research projects

Gene Modified Mesenchymal Stem Cell Therapy and Diagnosis

Due to the characteristic of WJ-MSCs, we hypothesis that WJ-MSCs can be a cell carrier to delivery multi-targeted vectors which may markedly enhance the therapeutic efficacy of glioblastoma.

 

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Molecular Image Application on Cancer

1. Bioluminescence Imaging

2. Micro Positron Emission Tomography

3. MR Chemical Exchange Saturation Transfer

 

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4. Magnetic resonance imaging (MRI)

 

Theranostic 

1. Molecular Magnetic Resonance Imaging (mMRI)

    (Superparamagnetic iron oxide based MRI contrast agents)

2. Drug delivery efficacy (covert by MR signal intensity)

 

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Molecular Image Application on Immunology

Anti-bodies based immunotherapy 

Bioluminescence Imaging​​​​

(Luminescence-based reporter gene model )

 

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1. Low dose chemo combined adoptive T transfer

2. Antibody combination treatment

 

CONTACT ME

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Phone

Email

Website

Address

+886-4-22053366#2532

sakiro920@gmail.com

https://1440114.wixsite.com/sakiro920

No.91 Hsueh-Shih Road, Taichung, Taiwan 40402

​(辦公室:水湳校區研究大樓7樓)

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Woei-Cheang Shyu

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Professor

Graduate Institute of Biomedical Sciences Professor

China Medical University

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Keng-Li Lan

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Associate Professor

Institute of Traditional Medicine

School of Medicine

National Yang-Ming University

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Chih-Chia Huang

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Professor

Department of Photonic

National Cheng-Kung University

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